Latest Articles
Research Article | Host-Microbe BiologyMetabolic Interactions between Brachypodium and Pseudomonas fluorescens under Controlled Iron-Limited ConditionsRhizosphere bacteria influence the growth of their host plant by consuming and producing metabolites, nutrients, and antibiotic compounds within the root system that affect plant metabolism. Under Fe-limited growth conditions, different plant and microbial species have distinct Fe acquisition strategies, often involving the secretion of strong Fe-binding chelators that scavenge Fe and facilitate uptake.
Research Article | Clinical Science and EpidemiologyEvolution of Ciprofloxacin Resistance-Encoding Genetic Elements in SalmonellaResistance of nontyphoidal Salmonella to fluoroquinolones such as ciprofloxacin is known to be mediated by target mutations. This study surveyed the prevalence of Salmonella strains recovered from 2,989 food products in Shenzhen, China, during the period 2012 to 2017 and characterized the genetic features of several PMQR gene-bearing plasmids and ciprofloxacin resistance-encoding DNA fragments.
Editor's Pick Research Article | Novel Systems Biology TechniquesA High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic ResistanceThe rapid transmission of antibiotic resistance genes on conjugative plasmids between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis. There are currently no experimental platforms for fast and cost-efficient screening of genetic effects on antibiotic resistance transmission by conjugation, which prevents understanding and targeting conjugation.
EditorialAcknowledgment of Ad Hoc Reviewers
Research Article | Molecular Biology and PhysiologyDistinct Community Assembly Processes of Abundant and Rare Soil Bacteria in Coastal Wetlands along an Inundation GradientCoastal wetlands are one of the important ecosystems that play a crucial role in the regulation of climate change. Rare taxa (RT) exist in one habitat along with abundant taxa (AT).
Research Article | Molecular Biology and PhysiologySingle-Cell RNA Sequencing Reveals that the Switching of the Transcriptional Profiles of Cysteine-Related Genes Alters the Virulence of Entamoeba histolyticaStudies on the trophozoite of Entamoeba histolytica suggested this organism could accumulate polyploid cells in its proliferative phase and differentiate its cell cycle from that of other eukaryotes. Therefore, a single-cell sequencing technique was used to study the switching of the RNA transcription profiles of single amoebic trophozoites.
Research Article | Molecular Biology and PhysiologySimultaneous Presence of Bacteriochlorophyll and Xanthorhodopsin Genes in a Freshwater BacteriumPhototrophic organisms are key components of many natural environments. There exist two main phototrophic groups: species that collect light energy using various kinds of (bacterio)chlorophylls and species that utilize rhodopsins.
Research Article | Applied and Environmental ScienceIsolation of SAR11 Marine Bacteria from Cryopreserved SeawaterHigh-throughput dilution culture has proved to be a successful approach to bring some difficult-to-isolate planktonic microorganisms into culture, including the highly abundant SAR11 lineage of marine bacteria. While the long-term preservation of bacterial isolates by freezing them in the presence of cryoprotectants, such as glycerol, has been shown to be an effective method of storing viable cells over long time periods (i.e., years),...
Research Article | Host-Microbe BiologyElucidating the Diversity and Potential Function of Nonribosomal Peptide and Polyketide Biosynthetic Gene Clusters in the Root MicrobiomeWe identified distinct secondary-metabolite-encoding genes that are enriched (relative to adjacent bulk soil) and expressed in root ecosystems yet almost completely absent in human gut and aquatic environments. Several of the genes were distantly related to genes encoding antimicrobials and siderophores, and their high sequence variability relative to known sequences suggests that they may encode novel metabolites and may have unique...
Editor's Pick Research Article | Host-Microbe BiologyTraditional Human Populations and Nonhuman Primates Show Parallel Gut Microbiome Adaptations to Analogous Ecological ConditionsThe results of this study highlight parallel gut microbiome traits in human and nonhuman primates, depending on subsistence strategy. Although these similarities have been reported before, the functional and ecological bases of this convergence are not fully understood.