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transposons

  • Open Access
    Comparative Genomic Analysis of <em>Mycobacteriaceae</em> Reveals Horizontal Gene Transfer-Mediated Evolution of the CRISPR-Cas System in the <span class="named-content genus-species" id="named-content-1">Mycobacterium tuberculosis</span> Complex
    Research Article | Ecological and Evolutionary Science
    Comparative Genomic Analysis of Mycobacteriaceae Reveals Horizontal Gene Transfer-Mediated Evolution of the CRISPR-Cas System in the Mycobacterium tuberculosis Complex

    Comparative genomic analysis of prokaryotes has led to a better understanding of the biology of several pathogenic microorganisms. One such clinically important pathogen is M. tuberculosis, the leading cause of bacterial infection worldwide.

    Anoop Singh, Mohita Gaur, Vishal Sharma, Palak Khanna, Ankur Bothra, Asani Bhaduri, Anupam Kumar Mondal, Debasis Dash, Yogendra Singh, Richa Misra
  • Open Access
    Magic Pools: Parallel Assessment of Transposon Delivery Vectors in Bacteria
    Research Article | Novel Systems Biology Techniques
    Magic Pools: Parallel Assessment of Transposon Delivery Vectors in Bacteria

    Molecular genetics is indispensable for interrogating the physiology of bacteria. However, the development of a functional genetic system for any given bacterium can be time-consuming. Here, we present a streamlined approach for identifying an effective transposon mutagenesis system for a new bacterium. Our strategy first involves the construction of hundreds of different transposon vector variants, which we term a “magic pool.” The...

    Hualan Liu, Morgan N. Price, Robert Jordan Waters, Jayashree Ray, Hans K. Carlson, Jacob S. Lamson, Romy Chakraborty, Adam P. Arkin, Adam M. Deutschbauer
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