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transposon mutagenesis

  • Open Access
    Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in <span class="named-content genus-species" id="named-content-1">Magnetospirillum gryphiswaldense</span>
    Research Article | Molecular Biology and Physiology
    Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in Magnetospirillum gryphiswaldense

    Magnetospirillum gryphiswaldense is one of the few tractable model magnetotactic bacteria (MTB) for studying magnetosome biomineralization. So far, knowledge on the genetic determinants of this complex process has been mainly gathered using reverse genetics and candidate approaches. In contrast, nontargeted forward genetics studies are lacking, since application of...

    Karen T. Silva, Margarete Schüler, Frank Mickoleit, Theresa Zwiener, Frank D. Müller, Ram Prasad Awal, Alfons Weig, Andreas Brachmann, René Uebe, Dirk Schüler
  • Open Access
    Development of a Counterselectable Transposon To Create Markerless Knockouts from an 18,432-Clone Ordered <span class="named-content genus-species" id="named-content-1">Mycobacterium bovis</span> Bacillus Calmette-Guérin Mutant Resource
    Editor's Pick Research Article | Molecular Biology and Physiology
    Development of a Counterselectable Transposon To Create Markerless Knockouts from an 18,432-Clone Ordered Mycobacterium bovis Bacillus Calmette-Guérin Mutant Resource

    While speeding up research for many fields of biology (e.g., yeast, plant, and Caenorhabditis elegans), genome-wide ordered mutant collections are still elusive in mycobacterial research. We developed methods to generate such resources in a time- and cost-effective manner and developed a newly engineered transposon from which unmarked mutants can be efficiently...

    Katlyn Borgers, Kristof Vandewalle, Annelies Van Hecke, Gitte Michielsen, Evelyn Plets, Loes van Schie, Sandrine Vanmarcke, Laurent Schindfessel, Nele Festjens, Nico Callewaert
  • Open Access
    Inferring the Minimal Genome of <em>Mesoplasma florum</em> by Comparative Genomics and Transposon Mutagenesis
    Research Article | Molecular Biology and Physiology
    Inferring the Minimal Genome of Mesoplasma florum by Comparative Genomics and Transposon Mutagenesis

    The last years have witnessed the development of whole-genome cloning and transplantation methods and the complete synthesis of entire chromosomes. Recently, the first minimal cell, Mycoplasma mycoides JCVI-syn3.0, was created. Despite these milestone achievements, several questions remain to be answered. For example, is the composition of minimal genomes virtually...

    Vincent Baby, Jean-Christophe Lachance, Jules Gagnon, Jean-François Lucier, Dominick Matteau, Tom Knight, Sébastien Rodrigue
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