SAR11
Research Article | Applied and Environmental ScienceIsolation of SAR11 Marine Bacteria from Cryopreserved SeawaterHigh-throughput dilution culture has proved to be a successful approach to bring some difficult-to-isolate planktonic microorganisms into culture, including the highly abundant SAR11 lineage of marine bacteria. While the long-term preservation of bacterial isolates by freezing them in the presence of cryoprotectants, such as glycerol, has been shown to be an effective method of storing viable cells over long time periods (i.e., years),...
Research Article | Ecological and Evolutionary ScienceThe Evolutionary Success of the Marine Bacterium SAR11 Analyzed through a Metagenomic PerspectiveAs the most abundant bacteria in oceans, the Pelagibacterales order (here SAR11) plays an important role in the global carbon cycle, but the study of the evolutionary forces driving its evolution has lagged considerably due to the inherent difficulty of obtaining pure cultures. Multiple evolutionary models have been proposed to explain the diversification of distinct lineages within a population; however, the identification of...
Research Article | Applied and Environmental ScienceMetagenome Mining Reveals Hidden Genomic Diversity of Pelagimyophages in Aquatic EnvironmentsSAR11 clade members are among the most abundant bacteria on Earth. Their study is complicated by their great diversity and difficulties in being grown and manipulated in the laboratory. On the other hand, and due to their extraordinary abundance, metagenomic data sets provide enormous richness of information about these microbes. Given the major role played by phages in the lifestyle and evolution of prokaryotic cells, the contribution...
Editor's Pick Research Article | Applied and Environmental ScienceWide Distribution of Phage That Infect Freshwater SAR11 BacteriaFonsibacter represents a significant microbial group of freshwater ecosystems. Although the genomic and metabolic features of these bacteria have been well studied, no phage infecting them has been reported. In this study, we reconstructed complete genomes of Fonsibacter and infecting phage and revealed their close relatedness to the phage infecting marine SAR11 members. Also, we illustrated that phage that infect ...
Research Article | Ecological and Evolutionary ScienceElemental Composition, Phosphorous Uptake, and Characteristics of Growth of a SAR11 Strain in Batch and Continuous CultureWhile SAR11 bacteria contribute a significant fraction to the total picoplankton biomass in the ocean and likely are major players in organic C and nutrient cycling, the cellular characteristics and metabolic features of most lineages have either only been hypothesized from genomes or otherwise not measured in controlled laboratory experimentation. The dearth of data on even the most basic characteristics for what is arguably the most...
Research Article | Ecological and Evolutionary ScienceReevaluating the Salty Divide: Phylogenetic Specificity of Transitions between Marine and Freshwater SystemsThe distribution of microbial diversity across environments yields insight into processes that create and maintain this diversity as well as potential to infer how communities will respond to future environmental changes. We integrated data sets from dozens of freshwater lake and marine samples to compare diversity across open water habitats differing in salinity. Our novel combination of sequence-based approaches revealed lineages that...
Research Article | Molecular Biology and PhysiologyProteome Remodeling in Response to Sulfur Limitation in “Candidatus Pelagibacter ubique”