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resuscitation

  • Open Access
    Proteomic Study of the Survival and Resuscitation Mechanisms of Filamentous Persisters in an Evolved <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> Population from Cyclic Ampicillin Treatment
    Research Article | Molecular Biology and Physiology
    Proteomic Study of the Survival and Resuscitation Mechanisms of Filamentous Persisters in an Evolved Escherichia coli Population from Cyclic Ampicillin Treatment

    Persisters are a subpopulation of cells with enhanced survival toward antibiotic treatment and have the ability to resume normal growth when the antibiotic stress is lifted. Although proteomics is the most suitable tool to study them from a system-level perspective, the number of persisters that present naturally is too few for proteomics analysis, and thus the complex mechanisms through which they are able to survive antibiotic...

    Jordy Evan Sulaiman, Henry Lam
  • Open Access
    16S rRNA/rRNA Gene Ratios and Cell Activity Staining Reveal Consistent Patterns of Microbial Activity in Plant-Associated Soil
    Research Article | Ecological and Evolutionary Science
    16S rRNA/rRNA Gene Ratios and Cell Activity Staining Reveal Consistent Patterns of Microbial Activity in Plant-Associated Soil

    Although the majority of microorganisms in natural ecosystems are dormant, relatively little is known about the dynamics of the active and dormant microbial pools through both space and time. The limited knowledge of microbial activity-dormancy dynamics is in part due to uncertainty in the methods currently used to quantify active taxa. Here, we directly compared two of the most common methods (16S ratios and active cell staining) for...

    Alan W. Bowsher, Patrick J. Kearns, Ashley Shade
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