profiling
Research Article | Ecological and Evolutionary ScienceUntangling Species-Level Composition of Complex Bacterial Communities through a Novel Metagenomic ApproachWe developed a novel method for accurate cataloguing of bacterial communities at (sub)species level involving amplification of the internal transcribed spacer (ITS) region through optimized primers, followed by next-generation sequencing and taxonomic classification of amplicons by means of a comprehensive database of bacterial ITS sequences. Host-associated, food, and environmental matrices were employed to test the performance of the...