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microbial genetics

  • Open Access
    Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in <span class="named-content genus-species" id="named-content-1">Magnetospirillum gryphiswaldense</span>
    Research Article | Molecular Biology and Physiology
    Genome-Wide Identification of Essential and Auxiliary Gene Sets for Magnetosome Biosynthesis in Magnetospirillum gryphiswaldense

    Magnetospirillum gryphiswaldense is one of the few tractable model magnetotactic bacteria (MTB) for studying magnetosome biomineralization. So far, knowledge on the genetic determinants of this complex process has been mainly gathered using reverse genetics and candidate approaches. In contrast, nontargeted forward genetics studies are lacking, since application of...

    Karen T. Silva, Margarete Schüler, Frank Mickoleit, Theresa Zwiener, Frank D. Müller, Ram Prasad Awal, Alfons Weig, Andreas Brachmann, René Uebe, Dirk Schüler
  • Open Access
    Consistent Metagenome-Derived Metrics Verify and Delineate Bacterial Species Boundaries
    Research Article | Ecological and Evolutionary Science
    Consistent Metagenome-Derived Metrics Verify and Delineate Bacterial Species Boundaries

    There is controversy about whether bacterial diversity is clustered into distinct species groups or exists as a continuum. To address this issue, we analyzed bacterial genome databases and reports from several previous large-scale environment studies and identified clear discrete groups of species-level bacterial diversity in all cases. Genetic analysis further revealed that quasi-sexual reproduction via horizontal gene transfer is...

    Matthew R. Olm, Alexander Crits-Christoph, Spencer Diamond, Adi Lavy, Paula B. Matheus Carnevali, Jillian F. Banfield
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