longitudinal
Research Article | Host-Microbe BiologyQuantifying Live Microbial Load in Human Saliva Samples over Time Reveals Stable Composition and Dynamic LoadHuman microbiomes are dynamic ecosystems often composed of hundreds of unique microbial taxa. To detect fluctuations over time in the human oral microbiome, we developed a novel workflow to quantify live microbial cells with flow cytometry in parallel with next-generation sequencing, and applied this method to over 150 unstimulated, timed saliva samples.
Research Article | Host-Microbe BiologyDevelopment of Inflammatory Bowel Disease Is Linked to a Longitudinal Restructuring of the Gut Metagenome in MiceIBD patients harbor distinct microbial communities with functional capabilities different from those seen with healthy people. But is this cause or effect? Answering this question requires data on changes in gut microbial communities leading to disease onset. By performing weekly metagenomic sequencing and mixed-effects modeling on an established mouse model of IBD, we identified several functional pathways encoded by the gut microbiome...