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ITS

  • Open Access
    Untangling Species-Level Composition of Complex Bacterial Communities through a Novel Metagenomic Approach
    Research Article | Ecological and Evolutionary Science
    Untangling Species-Level Composition of Complex Bacterial Communities through a Novel Metagenomic Approach

    We developed a novel method for accurate cataloguing of bacterial communities at (sub)species level involving amplification of the internal transcribed spacer (ITS) region through optimized primers, followed by next-generation sequencing and taxonomic classification of amplicons by means of a comprehensive database of bacterial ITS sequences. Host-associated, food, and environmental matrices were employed to test the performance of the...

    Christian Milani, Giulia Alessandri, Marta Mangifesta, Leonardo Mancabelli, Gabriele Andrea Lugli, Federico Fontana, Giulia Longhi, Rosaria Anzalone, Alice Viappiani, Sabrina Duranti, Francesca Turroni, Renato Costi, Alfredo Annicchiarico, Andrea Morini, Leopoldo Sarli, Maria Cristina Ossiprandi, Douwe van Sinderen, Marco Ventura
  • Open Access
    Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys
    Methods and Protocols | Novel Systems Biology Techniques
    Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

    We continue to uncover a wealth of information connecting microbes in important ways to human and environmental ecology. As our scientific knowledge and technical abilities improve, the tools used for microbiome surveys can be modified to improve the accuracy of our techniques, ensuring that we can continue to identify groundbreaking connections between microbes and the ecosystems they populate, from ice caps to the human body. It is...

    William Walters, Embriette R. Hyde, Donna Berg-Lyons, Gail Ackermann, Greg Humphrey, Alma Parada, Jack A. Gilbert, Janet K. Jansson, J. Gregory Caporaso, Jed A. Fuhrman, Amy Apprill, Rob Knight
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