TABLE 5

Functional validation of mutation detected in E. coli IMP-resistant mutants

TABLE 5
  • a Single knock-in, mutations in genes were transformed in an individual fashion; Double knock-in, mutations in two genes were transformed into the same E. coli ATCC 25922 cells. Gene IDs in bold had mutations in at least two E. coli and K. pneumoniae mutants (see Table 4). The gene in italics was mutated in E. coli, K. pneumoniae, and P. aeruginosa.

  • b The mutant whose genomic DNA was used to amplify the mutation by PCR to generate the knock-in cassettes.

  • c MICs were monitored with at least three biological replicates. For all differences of 2-fold or higher, there was no variability in the observed MICs. The kan resistance marker was removed from all transformants using the FLP/FLPe recombinase except for rpoD. ND, not determined.