TABLE 2

Sixteen experimentally characterized Acr-Aca loci in bacterial genomes

Bacterial speciesRefSeq genome IDPublished Acr-Aca lociPresence of complete CRISPR-Cas
and self-targeting spacer
Listeria monocytogenes J0161GCF_000168635AcrIIA2-AcrIIA1Yes
Listeria monocytogenes serotype 7 strain SLCC2482GCF_000210795AcrIIA2-AcrIIA3-AcrIIA1Yes
Listeria monocytogenes J0161GCF_000168635AcrIIA4-AcrIIA1Yes
Pseudomonas otitidisGCF_900111835AcrIE5-Aca1Yes
Pseudomonas aeruginosa WH-SGI-V-07059GCF_001450485aAcrIF4-AcrIE3-Aca1No self-target spacer
Haemophilus parainfluenzaeGCF_001053575aAcrIIC4-Aca2No self-target spacer
Pseudomonas citronellolisGCF_001654435aAcrIE4-IF7-Aca1No self-target spacer
Pseudomonas aeruginosa strain C 1426GCF_000412555aAcrIF11-Aca1No self-target spacer
Pseudomonas aeruginosa strain Jp54GCF_003836565bAcrIF6-Aca1No self-target spacer
Vibrio parahaemolyticusGCF_000736335aAcrIF9-Aca1No complete CRISPR-Cas
Shewanella xiamenensisGCF_000712635aAcrIF10-Aca1No complete CRISPR-Cas
Neisseria meningitidisGCF_001066195aAcrIIC1-Aca3No complete CRISPR-Cas
Neisseria meningitidis 992008GCF_000724735aAcrIIC2-AcaIIC3-Aca3No complete CRISPR-Cas
Simonsiella muelleri ATCC 29453GCF_000163775aAcrIIC5-Aca2No complete CRISPR-Cas
Pseudomonas aeruginosa strain S708_C14_RSGCF_002136415bAcrIE6-Aca1No complete CRISPR-Cas
Pseudomonas aeruginosa strain 359GCF_002312455bAcrIF12-Aca4No complete CRISPR-Cas
  • a These genomes are contained in the version of the RefSeq database that we have mined but have no self-targeting spacers according to the work of Watters et al. (12).

  • b These genomes were not in the version of the RefSeq database that we have mined, so we downloaded them separately from NCBI. All these genomes were analyzed using CRISPRCasFinder to confirm the presence of complete CRISPR-Cas systems and using our in-house programs to confirm the presence of self-targeting spacers.