TABLE 1

Among-fish variation greatly exceeds within-fish (among-protocol) variation for several diversity metrics, as indicated by high reproducibility estimatesa

Diversity variableAmong-fish variationWithin-fish variationReproducibility (95% CI)
Class richness77.40911.0800.875 (0.792, 0.917)
Class evenness0.0110.0010.920 (0.885, 0.965)
Species richness6606.324123.3930.982 (0.946, 0.991)
Species evenness0.0090.0010.923 (0.886, 0.945)
Phylogenetic diversity287.92918.9490.938 (0.870, 0.969)
Class Bray-Curtis0.0820.0040.958 (0.912, 0.979)
Class Bray-Curtis
(QIIME 2 with Deblur)
0.0810.0040.957 (0.921, 0.979)
Species Bray-Curtis0.1120.0040.966 (0.938, 0.982)
Weighted UniFrac0.0700.0030.955 (0.907, 0.978)
Weighted UniFrac
(QIIME 2 with Deblur)
0.0230.0010.959 (0.926, 0.978)
Unweighted UniFrac0.0560.1580.263 (0.207, 0.292)
Unweighted UniFrac
(QIIME 2 with Deblur)
0.0550.0460.542 (0.466, 0.583)
  • a Variance component and reproducibility estimates considering all individuals in experiment 1, along with bootstrap 95% confidence intervals (CIs) are shown. The reproducibility values are shown in boldface type.