TABLE 3

Disaccharides produced by the glycosynthase C11_GH1_E354S

DonorAcceptorProductYield (%)
α-F-GalpNP-GlcGal-(β-1,2)-Glc-β-pNP15
Gal-(β-1,3)-Glc-β-pNP20
α-F-GalpNP-α-XylGal-(β-1,2)-Xyl-α-pNP50
α-F-GalpNP-β-XylGal-(β-1,2)-Xyl-β-pNP60
6-N3-α-F-GalpNP-Glc6-N3-Gal-(β-1,4)-Glc-β-pNP23
6-N3-Gal-(β-1,2)-Glc-β-pNP21
6-N3-Gal-(β-1,3)-Glc-β-pNP37