TABLE 2

Kinetic constants determined for C11-GH1 on azido-modified and unmodified glycosides

SubstrateMean kcat (s−1)
± SD
Mean Km (mM)
± SD
Mean kcat/Km ratio
(mM−1 s−1) ± SD
Gal MU0.9 ± 0.20.05 ± 0.0220 ± 9
6-N3-Gal MU3.9 ± 0.50.10 ± 0.0240 ± 10
Glc MU63 ± 50.20 ± 0.03320 ± 50
6-N3-Glc MU14 ± 10.05 ± 0.01280 ± 60