TABLE 1

Impact of decreasing starting material for 16S rRNA gene sequencing

ParameterData by dilution
D0D1D2D3D4D5D6D7D8
No. of reads251,419172,915250,861247,581216,341136,081128,05341,07140,927
No. of unique ASVs1820114172262312381147193
No. of unique genera1516646180861075874
% contaminantsa0.10.11.84.512.027.964.555.880.1
  • a Percent contaminants calculated as the percentage of sequences in each sample that were not an exact match to the mock microbial community reference sequences.