TABLE 2

16S rRNA gene PCR primer sequences used in this study

RegionAmplicon size (bp)aNameDirectionPrimer sequence (5′–3′)
V1-V234927F-YMForwardAGAGTTTGATYMTGGCTCAG
338RReverseTGCTGCCTCCCGTAGGAGT
V3-V4466341FForwardCCTACGGGNGGCWGCAG
806RBReverseGGACTACNVGGGTWTCTAAT
V4292515FForwardGTGYCAGCMGCCGCGGTA
806RBReverseGGACTACNVGGGTWTCTAAT
  • a Mean amplicon size shown for hypervariable region amplified plus PCR primers.