TABLE 2

Genes differentially expressed by Y. pestis and the Y. pseudotuberculosis flea-transmissible mutant relative to the Y. pseudotuberculosis wild-type strain during infection of X. cheopis fleasa

Gene IDbGene namePredicted gene functionRelative expression fold differencec
IPmt:IPCO:IP
Day 1 after infection
    YPTB0570YPO0425hmsTDiguanylate cyclase, c-di-GMP synthesis2.32.4
    YPTB0592YPO0449hmsDDiguanylate cyclase, c-di-GMP synthesis2.35.1
    YPTB0728YPO3403panDAspartate alpha-decarboxylase2.52.5
    YPTB1108YPO2615ybeJGlutamate and aspartate transporter subunit2.02.4
    YPTB1923YPO1925evgTwo-component response regulator2.62.6
    YPTB3107YPO0863YaeF/YiiX family of permuted papain-like enzymes2.22.4
    YPTB3361YPO0700Fimbrial protein3.23.0
    YPTB3584YPO3646pcpOuter membrane lipoprotein SlyB3.42.6
    YPTB0338YPO0281hmuTABC transporter, periplasmic hemin-binding protein−4.9−3.7
    YPTB0339YPO0282hmuSHemin degradation/transport protein−6.6−5.0
    YPTB0612YPO0469dnaJChaperone protein−2.6−2.4
    YPTB1515YPO1499ABC transporter permease−3.4−2.3
    YPTB2113YPO2190Ail-family outer membrane protein−4.0−2.3
    YPTB2239YPO2320XRE family transcriptional regulator−2.4−4.0
    YPTB3475YPO0582YgjV family inner membrane protein−3.0−2.3
    YPTB3905YPO4084ibpBHeat shock chaperone−2.9−3.6
Day 14 after infection
    YPTB0728YPO3403panDAspartate alpha-decarboxylase2.52.5
    YPTB1107YPO2614gltJABC glutamate/aspartate transporter, permease subunit2.22.1
    YPTB1108YPO2615ybeJGlutamate and aspartate transporter subunit2.52.5
    YPTB1522YPO1507mglBGalactose binding periplasmic protein2.12.9
    YPTB1923YPO1925evgTwo-component response regulator3.23.9
    YPTB1934YPO1936Aminotransferase2.07.8
    YPTB1937YPO1939Glutaminase4.83.3
    YPTB1959YPO1962argDSuccinylornithine/acetylornithine transaminase2.22.2
    YPTB3107YPO0863YaeF/YiiX family of permuted papain-like enzymes2.52.1
    YPTB3361YPO0700Fimbrial protein2.12.4
    YPTB3534YPO3699PliI-like periplasmic lysozyme inhibitor2.39.7
    YPTB3582YPO36482-Hydroxy-3-oxopropionate reductase2.211.6
    YPTB3584YPO3646pcpOuter membrane lipoprotein SlyB3.53.2
    YPTB3736YPO0165LacI family transcriptional regulator2.12.0
    YPTB0110YPO3927argCN-Acetyl-gamma-glutamyl-phosphate reductase−5.0−3.6
    YPTB0111YPO3925argBAcetylglutamate kinase−3.0−3.0
    YPTB0336YPO0279hmuVHemin importer ATP-binding subunit−3.4−3.1
    YPTB0337YPO0280hmuUABC hemin transporter, permease subunit−4.1−3.6
    YPTB0338YPO0281hmuTABC transporter, periplasmic hemin-binding protein−6.8−6.1
    YPTB0339YPO0282hmuSHemin degradation/transport protein−9.5−7.9
    YPTB0526YPO3446argIOrnithine carbamoyltransferase subunit I−2.7−2.8
    YPTB1240YPO1200adiCArginine:agmatine antiporter−2.5−5.7
    YPTB1241YPO1201adiAArginine decarboxylase−2.0−4.6
    YPTB1423YPO1398cspBCold shock-like protein−3.4−3.0
    YPTB1515YPO1499ABC transporter permease−2.5−3.7
    YPTB1579YPO1570argGArgininosuccinate synthase−4.7−2.9
    YPTB2113YPO2190Ail-family outer membrane protein−2.4−4.4
    YPTB2174YPO2255araFArabinose binding periplasmic protein−2.0−2.0
    YPTB2954YPO2652asrAcid shock protein precursor−2.4−8.3
    YPTB3461YPO0598Putative hemolysin activator/exporter protein−5.0−5.0
    YPTB3856YPO4021Biotin carboxylase superfamily protein−2.8−3.1
  • a Genes indicated in bold type were differentially regulated on both day 1 and day 14 after infection.

  • b Data represent annotation numbers of the Y. pseudotuberculosis IP32953 (YPTB) and Y. pestis CO92 (YPO) homologs.

  • c Data represent fold differences in expression by Y. pseudotuberculosis mutant (IPmt) and Y. pestis (CO) relative to the Y. pseudotuberculosis wild-type (IP) strain during infection of fleas.