TABLE 1

Summary of sequencing results for vaginal samplesa

Library preparation method parameterResult by PCR type
1-step2-step2-step
No. of samples subjected to amplification929292
No. of samples successfully amplified498383
Sequencing platformMiSeqMiSeqHiSeq
Chimeric sequences detected (%)0.703.33.1
Mean no. (±SE) of nonchimeric, assembled sequences per sample11,080 ± 1,50614,282 ± 48350,514 ± 4,427
Median quality score per sample (Q1–Q3)36.2b (33.5–37.2)34.9b (29.9–36.3)37.1b (33.0–38.0)
  • a Figure S4 summarizes the prequality filtering per-cycle quality scores.

  • b Significant. Kruskal-Wallis, H = 187.85; P < 2.2 × 10−16.