TABLE 2

Sequencing run information for the MiSeq and HiSeq platforms

Sequencing platform parameterMiSeqHiSeq2500 RR
Run details2× 300 bp PE 2× 250 bp PE + 2× 50 bp PE
Mean no. (±SE) of assembled sequences per sample13,116 ± 47949,851± 895
No. of samples2761,194
Chimeric sequences detected (%)2.87.7
Mean no. (±SE) of nonchimeric, assembled sequences per sample12,737 ± 46345,988 ± 787
Median quality score (Q25–Q75), forward reads35.7a (33–37)36.1a (35–38)
Median quality score (Q25–Q75), reverse reads33.9b (25–36)33.2b (31–37)
Cost of sequencing per sample (no. of multiplexed samples)$6.38 (384)$3.99 (1,568)
  • a Significant. Wilcoxon rank sum, W = 70352; P < 2.2 × 10−16.

  • b Significant. Wilcoxon rank sum, W = 76453; P < 2.2 × 10−16.