TABLE 2

Summary of ETEC virulence factor content in the CS6- and CFA/I ST-only ETEC genomes

Virulence factorGenBank accession no. or sourceaNo. (%) of genomesbP valuec
All CFA/I
and CS6
CFA/ICS6
Toxins
    STIa (STp)YP_003294006.11 (<1)0 (0)1 (1)NS
    STIb (STh)WP_023485648.1242 (90)147 (91)95 (89)NS
    LT-IYP_003293996.1YP_003293997.14 (1)2 (1)2 (2)NS
    EAST1AAD43571.180 (30)37 (23)43 (40)0.0036
Colonization factors and adhesins
    CFA/ICBJ04486.1CBJ04489.1144 (54)143 (88)1 (1)<0.001
    CS6AAC45093.1AAC45096.189 (33)0 (0)89 (83)<0.001
    CS2CAA87760.1CAA87763.11 (<1)1 (<1)0 (0)NS
    CS3CAA34815.1CAA34820.11 (<1)1 (<1)0 (0)NS
    CS4AAK97134.1AAK97137.15 (2)0 (0)5 (5)0.0094
    CS5CAA11821.1CAA11825.157 (21)0 (0)57 (53)<0.001
    CS14AAQ20104.1AAQ20108.15 (2)3 (2)2 (2)NS
    CS21ABU50035.1ABU50050.1167 (62)142 (88)31 (29)<0.001
    CS22AAD30557.11 (<1)1 (<1)0 (0)NS
    EtpA (EtpBAC)AAX13508.1AAX13510.1152 (57)144 (89)8 (7)<0.001
    EatAAAO17297.1203 (75)137 (85)66 (62)<0.001
    SepA-likeThis study30 (11)2 (1)28 (26)<0.001
    SepAAAL72309.14 (1)2 (1)2 (2)NS
    TiaAAB06592.115 (6)13 (8)2 (2)NS
    TibACBJ01643.138 (14)34 (21)4 (4)<0.001
Other
    T2SSαCBJ03075.1CBJ03088.1156 (58)131 (81)25 (23)<0.001
    T2SSβCBJ02728.1CBJ02741.1246 (91)159 (98)87 (81)<0.001
  • a The GenBank accession numbers are indicated for the protein sequences.

  • b Data represent numbers of genomes that had each virulence factor with a BSR of ≥0.8, identified using both TBLASTN and BLASTN. The total numbers of genomes analyzed were as follows: all CFA/I and CS6, n = 269; CFA/I, n = 162; CS6, n = 107. The percentages of the genomes are indicated in parentheses.

  • c P values were generated by comparing the number of CFA/I genomes to the number of CS6 genomes that had each virulence factor by the chi square test (or Fisher's exact test when present in ≤5 genomes) using R v.3.4.1. NS, not significant (P value of >0.05).