TABLE 1

B. anthracis strains identified from the WGS screen as containing mutations within sigP, rsiP, and bla1a

StrainGenent mutation
position(s)
Confirmed by
Sanger
sequencing
Mutation
type
Predicted effectPEN MIC
(µg/ml)
Mutation
previously
described
(reference[s])
2002013094sigP119YesSNP; transition(G) ▶ (D)≤0.015No
2000031021sigP119YesSNP; transition(G) ▶ (D)0.3No
2000031052sigP119, 395YesSNP; transitions(G) ▶ (D), (L) ▶ (S)0.3No
2002734089bla193YesSNP; transitionSilent mutation (G) ▶ (G)0.06No
2000031048bla1−64†YesSNP; transversion in −35
binding element
5′-ATGGAACAAA-3′ ▶
5′-ATGGAAAAAA-3′
0.3No
2002013017rsiP60NoSNP; transversion(H) ▶ (Q)≤0.015No
2002013007rsiP827NoInsertion of AAAAAGDeletion of (K) + (R)0.03No
2002013011rsiP505YesSNP; transition(Y) ▶ (H)0.03No
2000031038rsiP39YesSNP; deletionFS; truncates RsiP to 30 aa>512No
2000032823rsiP471YesSNP; insertionFS; truncates RsiP to 163 aa256Yes (18)
2002734065rsiP10YesSNP; deletionFS; truncates RsiP to 12 aa>512Yes (4)
2002734039rsiP10YesSNP; deletionFS; truncates RsiP to 12 aa>512Yes (4)
2002013027rsiP10YesSNP; deletionFS; truncates RsiP to 12 aa0.06Yes (4)
Pen-R strains not
included in screen*
    32sigP183YesSNP; transition(D) ▶ (G)>512Yes (4, 15)
rsiP10YesSNP; deletionFS; truncates RsiP to 12 aa>512Yes
    UT308sigP183YesSNP; transition(D) ▶ (G)>512Yes (4, 15)
rsiP10YesSNP; deletionFS; truncates RsiP to 12 aa>512Yes (4, 15)
    SK57rsiP10YesSNP; deletionFS; truncates RsiP to 12 aa>512Yes
  • a Nucleotide (nt) mutation positions representing the position within the corresponding gene in the Ames Ancestor reference sequence (NC_007530.2). The last column indicates whether the corresponding mutation has been previously described in the literature. Amino acid abbreviations are as follows: (G), glycine; (D), aspartic acid; (L), leucine; (S), serine, (H), histidine; (Q), glutamine; (Y), tyrosine. †, nt position upstream of the ATG start codon for bla1. *, penicillin-resistant strains not included in this screen but included in this table for reference purposes. FS, frameshift.