TABLE 2

Strains and plasmids for bacitracin MIC measurement

Strain or plasmidDescriptionReference
Strains
    E. coli
        SM10thi thr leu tonA lacY supE recA RP4-2-Tc::Mu (Km)64
        BYF684SM10 harboring pYF684This study
        S17.1thi pro hsdR RP4-2-Tc::Mu Km::Tn7 (Tp Sm)64
        BYF701S17.1 harboring pRP1099This study
        BYF794S17.1 harboring pYF794This study
        BYF796S17.1 harboring pYF796This study
        BYF797S17.1 harboring pYF797This study
        BYF798S17.1 harboring pYF798This study
        BYF799S17.1 harboring pYF799This study
        BYF800S17.1 harboring pYF800This study
        BYF801S17.1 harboring pYF801This study
        BYF802S17.1 harboring pYF802This study
        BYF803S17.1 harboring pYF803This study
    B. anthracis
        34F2B. anthracis vaccine strain62
        BYF1002834F2 with the region between rrnG and rrnH deleted (DelGH)This study
        BYF10031BYF10028 harboring pYF794This study
        BYF10033BYF10028 harboring pYF798This study
        BYF10034BYF10028 harboring pYF799This study
        BYF10035BYF10028 harboring pYF800This study
        BYF10036BYF10028 harboring pYF801This study
        BYF10037BYF10028 harboring pYF796This study
        BYF10038BYF10028 harboring pYF797This study
        BYF10039BYF10028 harboring pYF802This study
        BYF10040BYF10028 harboring pYF803This study
Plasmids
    pRP1028Vector for introduction of I-SceI recognition site61
    pYF684pRP1028 cloned with sequence around bcrB of 34F2This study
    pRP1099Vector for introduction of the gene encoding I-SceI61
    pYF794pRP1099 without I-SceI and AmCyanThis study
    pYF796pYF794 with cloned uppP, bcrB, and bcrA of 34F2This study
    pYF797pYF794 with cloned bcrB and bcrA of 34F2This study
    pYF798pYF794 with cloned bcrA of 34F2This study
    pYF799pYF794 with cloned bacS and bacR of 34F2This study
    pYF800pYF794 with cloned bacR of 34F2This study
    pYF801pYF794 with cloned uppP of 34F2This study
    pYF802pYF794 with cloned bcrB of 34F2This study
    pYF803pYF794 with cloned bacS of 34F2This study