TABLE 1

Tn mutants identified by SMarT TnSeq as significantly underrepresented in the presence of cholic acida

PositionLocus tagGeneGene productLog2 fold change (adjusted P value)
Cholic acid
(t1)/input
Cholic acid
(t1)/untreated (t1)
Cholic acid
(t2)/input
Cholic acid
(t2)/untreated (t2)
45155OG1RF_10039radADNA repair protein RadA−0.71 (1.89E−39)−0.87 (2.81E−24)−2.12 (6.68E−141)−2.17 (NS)
45325OG1RF_10039radADNA repair protein RadA−0.64 (1.82E−41)−1.12 (1.55E−21)−2.33 (1.05E−158)−2.54 (6.45E−8)
266158OG1RF_10257ezrASeptation ring
formation regulator
EzrA
−0.80 (1.02E−42)−0.64 (1.17E−22)−2.34 (8.33E−108)−2.30 (NS)
665366OG1RF_10627aadAldehyde-alcohol
dehydrogenase
−1.47 (9.81E−6)−0.92 (9.18E−13)−2.29 (1.19E−54)−1.61 (5.93E−7)
665450OG1RF_10627aadAldehyde-alcohol
dehydrogenase
−1.61 (NS)−0.48 (0.033)−2.18 (5.39E−11)−1.17 (6.64E−28)
1118301Intergenic_1127−2.06 (NS)−1.34 (NS)−2.29 (1.45E−10)−0.81 (0.0026)
1118585OG1RF_11076hrcAHeat-inducible transcription
repressor HrcA
−2.77 (NS)−1.84 (5.38E−37)−2.72 (3.92E−23)−2.01 (5.29E−15)
1118707OG1RF_11076hrcAHeat-inducible transcription
repressor HrcA
−2.76 (NS)−1.43 (8.98E−16)−3.47 (1.77E−13)−2.48 (1.6E−21)
1119789OG1RF_11077grpECochaperone GrpE−2.08 (NS)−0.59 (1.82E−11)−3.22 (4.01E−9)−2.50 (1.09E−22)
1120304OG1RF_11078dnaKChaperone DnaK−1.83 (NS)−1.44 (4.07E−17)−2.40 (8.07E−19)−2.21 (NS)
1121988Intergenic_1130−1.89 (NS)−1.24 (1.31E−16)−2.76 (8.1E−33)−2.16 (2.04E−5)
1122948OG1RF_11080dnaJChaperone DnaJ−2.11 (NS)−1.60 (5.16E−10)−2.42 (5.01E−22)−1.67 (NS)
1213789OG1RF_11160NAThioesterase−1.12 (1.9E−21)0.60 (NS)−2.98 (3.05E−5)−1.10 (1.16E−48)
1496094OG1RF_11434lspASignal peptidase II−1.33 (2.05E−5)−0.36 (NS)−3.06 (5.66E−12)−1.89 (0.025)
1497213Intergenic_1490−0.90 (1.74E−8)−0.33 (2.5E−4)−2.02 (8.52E−19)−1.79 (0.0034)
1707088OG1RF_11636relAGTP diphosphokinase−0.85 (8.27E−4)−0.094 (NS)−2.76 (3.37E−4)−2.37 (NS)
1793746OG1RF_11714NAGroup 2
glycosyltransferase
−0.82 (6.87E−9)−0.059 (NS)−2.31 (7.44E−10)−1.62 (0.0051)
1955393OG1RF_11854czrAMetal transport repressor
protein CzrA
−0.96 (6.74E−8)−0.44 (NS)−2.11 (4.8E−22)−1.54 (NS)
1955400OG1RF_11854czrAMetal transport repressor
protein CzrA
−1.23 (4.48E−6)−0.40 (NS)−2.36 (2.89E−16)−1.80 (1.11E−9)
2099505OG1RF_11987atpGATP synthase F1 sector
gamma subunit
−2.13 (4.14E−6)−0.85 (2.48E−11)−3.27 (5.25E−37)−2.08 (8.67E−45)
2274738OG1RF_12155NABrp/Blh family beta-
carotene 15,15-
monooxygenase
−1.08 (1.00E−4)−1.27 (5.73E−25)−2.05 (1.15E−63)−2.15 (NS)
2274991OG1RF_12155NABrp/Blh family beta-
carotene 15,15-
monooxygenase
−2.20 (NS)−2.17 (1.17E−56)−2.75 (3.91E−93)−2.49 (NS)
2275412OG1RF_12155NABrp/Blh family beta-
carotene 15,15-
monooxygenase
−1.12 (3.41E−18)−1.20 (3.68E−26)−2.10 (1.88E−145)−1.82 (NS)
2616803OG1RF_12481NACholine binding protein1.10 (NS)−1.13 (NS)−2.87 (1.34E−8)−3.60 (NS)
2696898OG1RF_12538guaBIMP dehydrogenase−1.58 (NS)−1.24 (5.24E−5)−2.10 (4.36E−27)−1.41 (NS)
2731478OG1RF_12569gcdBGlutaconyl-CoA
decarboxylase
−1.21 (6.05E−5)−0.49 (NS)−2.90 (8.84E−14)−1.98 (NS)
  • a The log2 fold changes are in relative abundance between the two indicated conditions. For adjusted P values, an adjusted P  of < 0.05 is significant; NS indicates “not significant.” Boldface values indicate the most extremely underrepresented mutants (log2 fold changes less than −2). The position column indicates the first nucleotide position of the Tn insertion site. NA, not assigned.