TABLE 1

Comprehensive summary of g-1 HEV and human cDNA library screeninga

Viral baitLTHALTHA +
Ar+ +
X α-Gal
LT +
X α-Gal
(3 times)
No. of
plasmids
Unique
restriction
pattern
No. of colonies after
retransformation
Unique coding
sequence in
frame with BD
Fetal brain cDNA library screening
    Met4335302916104
    Y1000000
    PCP8950272521122
    V2371127131753
    X965754521185
    Hel13110000
    RdRp300b300c25522517414021
    ORF25221111
    ORF333018011091654411
    ORF414413510810562508
Liver cDNA library screening
    Met105503227232215
    Y38777444
    PCP18095807627113
    V23315014011724126
    X7457575220176
    Hel11723222220129
    RdRp244b222c2031991126020
    ORF215886621
    ORF4302209164159895518
  • a BD, binding domain; L, leucine; T, tryptophan; H, histidine; A, adenine; Ar+, aureobasidin A; α-Gal, alpha-galactosidase; −, deficiency in the medium; +, supplemented in the medium.

  • b For RdRp, after mating cells were plated on LTHA + 10 mM 3-AT (3-amino 1,2,4 triazole).

  • c For RdRp, 10 mM 3-AT was included in the LTHA + Ar+ + X α-Gal.