TABLE 1

Summary statistics for full mutant librariesa

Category and characteristic
or parameter
Sphingo3Brev2Cola PontiPedo557
Summary of mutant libraries
    Mutant library nameSphingo3_ML4Brev2_ML6Cola_ML5Ponti_ML7Pedo557_ML3
    Transposonmariner vector
(pTGG37_NN1)
mariner vector
(pTGG39_NN1)
mariner vector
(pTGG43_NN2)
mariner vector
(pTGG43_NN2)
mariner vector
(pTGG43_NN2)
    Method of deliveryConjugationConjugationConjugationConjugationConjugation
    No. of strains with unique barcodesb275,037166,981278,255190,948190,892
    % of barcodes with intact vectorsc<0.01<0.01<0.01<0.01<0.01
Protein-coding genes
    Total no.4,9033,2104,6254,5465,229
    No. with central insertionsd3,9432,5844,0904,0104,670
    Median no. of strains/genee3027321921
    Insertion bias (%)f50.649.948.249.648.8
    Mean/median no. of reads/geneg1.601.681.491.421.40
BarSeq statistics
    % of reads with barcode9898989898
    % of barcodes that map to a straing6075617380
    No. of reads/million for the median geneg,h79128737990
  • a The organisms are abbreviated as follows: Sphingo3, Sphingobium sp. strain GW456-12-10-14-TSB1; Brev2, Brevundimonas sp. strain GW460-12-10-14-LB2; Cola, Echinicola vietnamensis DSM17526; Ponti, Pontibacter actiniarum DSM19842; Pedo557, Pedobacter sp. strain GW460-11-11-14-LB5.

  • b Only strains with insertions in the genome are included.

  • c May represent integration events of the entire transposon plasmid into the genome.

  • d When the transposon lies within the central 10 to 90% of a gene.

  • e Includes only protein-coding genes with at least one central insertion.

  • f Insertion bias toward coding strand of genes.

  • g Computed with time zero samples.

  • h Includes reads that have no multiplex or barcode in the denominator.