TABLE 1

Strains used in this study

StrainRelevant genotypeSourcea
B. subtilis
    BD630his leu8 metB5Lab strain
    BD3955his leu8 metB5 amyE::Pxyl-mreBCD Spc ΔmreB::KanThis study
    BD7587his leu8 metB5 mreB-K240QThis study
    BD7619his leu8 metB5 mreB-K240RThis study
E. coli
    ED1881Stellar/pminimad2-mreBCD AmpThis study
    ED1888Stellar/pminimad2-mreBCD-K240R AmpThis study
    ED1895Stellar/pminimad2-mreBCD-K240Q AmpThis study
  • a The Pxyl-mreBCD and ΔmreB::Kan constructs were kindly provided by R. Daniels and J. Errington.