TABLE 1

Predicted binding sites for SCO0204 (OsdR) in Streptomyces coelicolor

Locus
taga
FunctionSequencePositionbScorecCotranscribed
gene(s)d
Function(s)
SCO0204cOsdR, two-component response
regulator
AGGGCCGGTCGGCCCC−8113.74
SCO0200cUniversal stress proteinGGGGCCGACCGTCCCT−10012.49SCO0199c/
SCO0198c
Zinc-deprived alcohol dehydrogenase,
universal stress protein
SCO0215cNitroreductase family proteinAGGGCCGTCCGGCCCC−9912.24
SCO0208Pyruvate phosphate dikinaseCGGGCCGACCGGCCCT−10212.19
−158
SCO0207cUniversal stress protein−14410.08
−88
SCO5979Enoyl-CoA hydrataseCGGGACCTTCGGCCCT−8011.62SCO5980Bifunctional hydroxylase,
oxidoreductase
SCO5978cHypothetical protein−68
SCO2637Secreted serine proteaseAGGGCCGGTCGGCCTT−5311.27
SCO7188cSubtilisin-like secreted peptidaseGGGGACGATCGTCCCC−4711.2
SCO0039Hypothetical proteinAGGCCCGTTCCGCCCT−13210.86SCO0040/
SCO0041/
SCO0042
Glycosyl transferase, integral
membrane protein,
hypothetical protein
SCO0038cSigma factor−130SCO0037c/
SCO0036c
Sigma factor, hypothetical protein
SCO0168Crp-like regulatory proteinGAGGCCGGTCGGCCCT−28410.75
GGGGCCGACGGTCCCT−369.21
SCO0167cUniversal stress proteinAGGGACCTTCGGCCCC−39110.75
−11410.73
SCO0216Nitrate reductase alpha chain NarG2AGGGACCTTCGGCCCC−5310.73SCO0217/
SCO0218/
SCO0219
Nitrate reductase beta chain NarH2,
nitrate reductase delta chain NarJ2,
nitrate reductase delta chain NarI2
SCO5410Hypothetical proteinAGGGCAGGACGGCCCT+3610.6
SCO6041Protoporphyrinogen oxidaseGGGGCCGTCCGGCCCC−5110.57SCO6042Chlorite dismutase (oxygen-generating
enzyme)
SCO6040cLipoprotein−246SCO6039cFlavoprotein oxidoreductase, CoA
disulfide reductase
SCO3431EmrB/QacA subfamily transporterGGGGCCGAACGGCCGT+1310.52
SCO6164Hypothetical protein with DksA/TraR
family C4 zinc finger domain
GGGTCCGATCGGCCCG−6210.5
SCO6163cSensor kinase−334SCO6162cTwo-component system response
regulator
SCO0517Possible Crp-like regulatory proteinGGGACCGACCGGCCCT−24810.49
AGGGCCGGCCGGCCCG−26810.46
SCO3857Nosiheptide resistance regulatorGGGCCCGTTCGGCGCT−27110.34
SCO3856cPeptidyl-prolyl cis-trans isomerase−66
SCO5251Puromycin N-acetyltransferaseAGGGCCGTACGGCACC−24310.31
SCO2347Integral membrane proteinAGGGCCGAAAGTCCCG−29510.3
SCO2348Secreted protein−221
SCO0214Pyridoxamine 5′-phosphate oxidaseGGGGCCATCCGGCCCT−5010.18
SCO0213cNitrate-nitrite transporter protein−252SCO0212cHemerythrin cation binding
domain-containing protein
(oxygen transporting protein)
SCO0179cZinc-containing dehydrogenaseTGGGCCGGTCGGCCCC−1529.46
SCO7021Secreted proteinAGGCCCGAACGGCCCA−949.4SCO7022Hypothetical protein SC1H10.11.
SCO4412Regulatory proteinAGGGCGGAACGGCCGT−2619.34
SCO0355Conserved hypothetical protein
SCF41.14
AGGGCTGACCGGCCCG−819.2SCO0356Probable oxidoreductase
  • a SCO numbers in boldface were tested by EMSA.

  • b Position relative to the start of the gene.

  • c The cutoff score calculated using the PREDetector algorithm and based on the position weight matrix in Table S3 in the supplemental material.

  • d Genes known or predicted to be cotranscribed with the gene and therefore likely influenced by the regulatory element.