TABLE 2

RT-PCR verification of gene array dataa

GeneSignal for strain in presence of Zn or EDTA
Δ7ΔzupT mutantAE104
ZnEDTAZnEDTAZnEDTA
rpoZ (control)++++++++++++
Rmet_1098, cobW1/++/+++/+
Rmet_0837/++/++/+(+)
Rmet_5640/(+)///(+)
Rmet_5890, feoB/+(+)+++++(+)
Rmet_1533, hoxN//++++(+)(+)
Rmet_1794////+(+)
  • a To cross-check some gene array data points with an independent method, the presence of the mRNAs for six genes was semiquantified using RT-PCR. Cells of AE104, ΔzupT, and Δ7 strains were incubated in TMM in the presence of 10 µM Zn(II) or 50 µM EDTA, and RNA was isolated, reverse transcribed, and amplified by PCR. Three experiments with a positive control of DNA and a negative control with water were performed. /, no signal; other symbols represent signal strength decreasing from +++ and ++ via +(+) and + to (+), which represents a weak signal.