TABLE 1

PCR primer sequences used in this study (old and new constructs)

Primer namePrimer sequenceaReference
515f OriginalGTGCCAGCMGCCGCGGTAACaporaso et al. (1)
806r OriginalGGACTACHVGGGTWTCTAATCaporaso et al. (1)
515f ModifiedGTGYCAGCMGCCGCGGTAAParada et al. (4)
806r ModifiedGGACTACNVGGGTWTCTAATApprill et al. (5)
926rCCGYCAATTYMTTTRAGTTTParada et al. (4)
ITS1fCTTGGTCATTTAGAGGAAGTAAGardes and Bruns (6)
ITS2GCTGCGTTCTTCATCGATGCWhite et al. (7)
  • a Primers are listed in a 5′-to-3′ orientation. The original 515f and 806r primers are listed for comparison to the added degeneracy in the new constructs (changes are shown in bold). See Smith and Peay (8) for further details on the modifications to the ITS sequencing primers.