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Methods and Protocols | Novel Systems Biology Techniques

Impact of Preservation Method and 16S rRNA Hypervariable Region on Gut Microbiota Profiling

Zigui Chen, Pak Chun Hui, Mamie Hui, Yun Kit Yeoh, Po Yee Wong, Martin C. W. Chan, Martin C. S. Wong, Siew C. Ng, Francis K. L. Chan, Paul K. S. Chan
J. Gregory Caporaso, Editor
Zigui Chen
aCentre for Gut Microbiota Research, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
bDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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  • ORCID record for Zigui Chen
Pak Chun Hui
bDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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Mamie Hui
aCentre for Gut Microbiota Research, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
bDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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Yun Kit Yeoh
aCentre for Gut Microbiota Research, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
bDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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Po Yee Wong
bDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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Martin C. W. Chan
bDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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  • ORCID record for Martin C. W. Chan
Martin C. S. Wong
aCentre for Gut Microbiota Research, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
cJockey Club School of Public Health and Primary Care, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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Siew C. Ng
aCentre for Gut Microbiota Research, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
dDepartment of Medicine and Therapeutics, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
eLKS Institute of Health Science, State Key Laboratory of Digestive Disease, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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Francis K. L. Chan
aCentre for Gut Microbiota Research, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
dDepartment of Medicine and Therapeutics, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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Paul K. S. Chan
aCentre for Gut Microbiota Research, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
bDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
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J. Gregory Caporaso
Northern Arizona University
Roles: Editor
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DOI: 10.1128/mSystems.00271-18
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Article Information

DOI 
https://doi.org/10.1128/mSystems.00271-18

Published By 
American Society for Microbiology Journals
History 
  • Received October 28, 2018
  • Accepted February 1, 2019
  • Published online February 26, 2019.

Copyright & Usage 
Copyright © 2019 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • J. Gregory Caporaso, Editor, Northern Arizona University

Author Information

  1. Zigui Chena,b,
  2. Pak Chun Huib,
  3. Mamie Huia,b,
  4. Yun Kit Yeoha,b,
  5. Po Yee Wongb,
  6. Martin C. W. Chanb,
  7. Martin C. S. Wonga,c,
  8. Siew C. Nga,d,e,
  9. Francis K. L. Chana,d and
  10. Paul K. S. Chana,b
  1. aCentre for Gut Microbiota Research, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
  2. bDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
  3. cJockey Club School of Public Health and Primary Care, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
  4. dDepartment of Medicine and Therapeutics, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
  5. eLKS Institute of Health Science, State Key Laboratory of Digestive Disease, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China
  • Address correspondence to Zigui Chen, zigui.chen{at}cuhk.edu.hk, or Paul K. S. Chan, paulkschan{at}cuhk.edu.hk.
  • Citation Chen Z, Hui PC, Hui M, Yeoh YK, Wong PY, Chan MCW, Wong MCS, Ng SC, Chan FKL, Chan PKS. 2019. Impact of preservation method and 16S rRNA hypervariable region on gut microbiota profiling. mSystems 4:e00271-18. https://doi.org/10.1128/mSystems.00271-18.

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Impact of Preservation Method and 16S rRNA Hypervariable Region on Gut Microbiota Profiling
Zigui Chen, Pak Chun Hui, Mamie Hui, Yun Kit Yeoh, Po Yee Wong, Martin C. W. Chan, Martin C. S. Wong, Siew C. Ng, Francis K. L. Chan, Paul K. S. Chan
mSystems Feb 2019, 4 (1) e00271-18; DOI: 10.1128/mSystems.00271-18

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Impact of Preservation Method and 16S rRNA Hypervariable Region on Gut Microbiota Profiling
Zigui Chen, Pak Chun Hui, Mamie Hui, Yun Kit Yeoh, Po Yee Wong, Martin C. W. Chan, Martin C. S. Wong, Siew C. Ng, Francis K. L. Chan, Paul K. S. Chan
mSystems Feb 2019, 4 (1) e00271-18; DOI: 10.1128/mSystems.00271-18
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KEYWORDS

16S rRNA gene
amplicon sequencing
bacterial culture
gut microbiota
preservative

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