Methods and Protocols | Novel Systems Biology Techniques

Direct PCR Offers a Fast and Reliable Alternative to Conventional DNA Isolation Methods for Gut Microbiomes

Elin Videvall, Maria Strandh, Anel Engelbrecht, Schalk Cloete, Charlie K. Cornwallis
Chaysavanh Manichanh, Editor
DOI: 10.1128/mSystems.00132-17

Article Information

DOI 
https://doi.org/10.1128/mSystems.00132-17
PubMed 
29181448
Published By 
American Society for Microbiology Journals
History 
  • Received September 28, 2017
  • Accepted October 30, 2017
  • Published online November 21, 2017.
Copyright & Usage 
Copyright © 2017 Videvall et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license .
Editors and / or Reviewers 
  • Chaysavanh Manichanh, Editor, Vall d'Hebron Research Institute

Author Information

  1. Elin Videvalla,
  2. Maria Strandha,
  3. Anel Engelbrechtb,
  4. Schalk Cloeteb,c and
  5. Charlie K. Cornwallisa
  1. a Department of Biology, Lund University, Lund, Sweden
  2. b Western Cape Department of Agriculture, Directorate Animal Sciences, Elsenburg, South Africa
  3. c Department of Animal Sciences, Stellenbosch University, Matieland, South Africa
  • Address correspondence to Elin Videvall, elin.videvall{at}biol.lu.se, or Maria Strandh, maria.strandh{at}biol.lu.se.

  • E.V. and M.S. contributed equally to this work.

  • Citation Videvall E, Strandh M, Engelbrecht A, Cloete S, Cornwallis CK. 2017. Direct PCR offers a fast and reliable alternative to conventional DNA isolation methods for gut microbiomes. mSystems 2:e00132-17. https://doi.org/10.1128/mSystems.00132-17 .

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KEYWORDS

16S rRNA
DNA extraction
direct PCR
library preparation
microbiota
repeatability

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