Novel Systems Biology Techniques
Opinion/Hypothesis | Novel Systems Biology TechniquesRepurposing Didanosine as a Potential Treatment for COVID-19 Using Single-Cell RNA Sequencing DataAs of today (7 April 2020), more than 81,000 people around the world have died from the coronavirus disease 19 (COVID-19) pandemic. There is no approved drug or vaccine for COVID-19, although more than 10 clinical trials have been launched to test potential drugs. In an urgent response to this pandemic, I developed a bioinformatics pipeline to identify compounds and drug candidates to potentially treat COVID-19. This pipeline is based...
Research Article | Novel Systems Biology TechniquesMultiple-Disease Detection and Classification across Cohorts via Microbiome SearchHere, we present a search-based strategy for disease detection and classification, which detects diseased samples via their outlier novelty versus a database of samples from healthy subjects and then compares them to databases of samples from patients. This approach enables the identification of microbiome states associated with disease even in the presence of different cohorts, multiple sequencing platforms, or significant...
Editor's Pick Research Article | Novel Systems Biology TechniquesSystematic Localization of Escherichia coli Membrane ProteinsCurrent knowledge of protein localization, particularly outer membrane proteins, is highly dependent on bioinformatic predictions. To date, no systematic experimental studies have directly compared protein localization spanning the inner and outer membranes of E. coli. By combining sucrose density gradient fractionation of inner membrane (IM) and outer membrane (OM)...
Methods and Protocols | Novel Systems Biology Techniquesmanta: a Clustering Algorithm for Weighted Ecological Networksmanta comes with unique strengths, such as the abilities to identify nodes that represent an intermediate between clusters, to exploit negative edges, and to assess the robustness of cluster membership. manta does not require parameter tuning, is straightforward to install and run, and can be easily combined with existing microbial network inference tools.
Research Article | Novel Systems Biology TechniquesToward Standards in Clinical Microbiota Studies: Comparison of Three DNA Extraction Methods and Two Bioinformatic PipelinesMethod choice throughout the workflow of a microbiome study, from sample collection to DNA extraction and sequencing procedures, can greatly affect results. This study evaluated three different DNA extraction methods and two bioinformatic pipelines by including positive and negative controls and various biological specimens. By identifying an optimal combination of DNA extraction method and bioinformatic pipeline use, we hope to...
Research Article | Novel Systems Biology TechniquesThe Signal and the Noise: Characteristics of Antisense RNA in Complex Microbial CommunitiesThis study systematically evaluated the global patterns of microbial antisense expression across various environments and provides a bird’s-eye view of general patterns observed across data sets, which can provide guidelines in our understanding of antisense expression as well as interpretation of metatranscriptomic data in general. This analysis highlights that in some environments, antisense expression from microbial communities can...
Research Article | Novel Systems Biology TechniquesGeometry-Aware Cell Detection with Deep LearningAutomated diagnostic microscopy powered by deep learning is useful, particularly in rural areas. However, there is no general method for object detection of different cells. In this study, we developed GFS-ExtremeNet, a geometry-aware deep-learning method which is based on the detection of four extreme key points for each object (topmost, bottommost, rightmost, and leftmost) and its center point. A postprocessing step, namely, adjacency...
Research Article | Novel Systems Biology TechniquesTransfer Learning for Toxoplasma gondii RecognitionToxoplasma gondii, one of the world’s most common parasites, can infect all types of warm-blooded animals, including one-third of the world’s human population. Artificial intelligence (AI) could provide accurate and rapid diagnosis in fighting Toxoplasma. So far, none of the previously reported deep learning methods have attempted to explore the advantages of...
Research Article | Novel Systems Biology TechniquesExtreme Antibiotic Persistence via Heterogeneity-Generating Mutations Targeting TranslationBacterial persistence is a fascinating phenomenon in which a small subpopulation of bacteria becomes phenotypically tolerant to lethal antibiotic exposure. There is growing evidence that populations of bacteria in chronic clinical infections develop a hyperpersistent phenotype, enabling a substantially larger subpopulation to survive repeated antibiotic treatment. The mechanisms of persistence and modes of increasing persistence rates...
Research Article | Novel Systems Biology TechniquesMICOM: Metagenome-Scale Modeling To Infer Metabolic Interactions in the Gut MicrobiotaThe bacterial communities that live within the human gut have been linked to health and disease. However, we are still just beginning to understand how those bacteria interact and what potential interventions to our gut microbiome can make us healthier. Here, we present a mathematical modeling framework (named MICOM) that can recapitulate the growth rates of diverse bacterial species in the gut and can simulate metabolic interactions...