Article Figures & Data
Figures
- FIG 1
The successional dynamics of microbial consortia. Differences in microbial community structure and alpha diversity are plotted with respect to native soil communities (labeled as “n”), the 6-week-chitin-enriched communities (labeled as “c”), uninoculated control (labeled as “control”), and different serial dilutions of NAG enrichment in liquid and soil treatments for 0 to 15 weeks. The results are partitioned by initial dilution and incubating conditions (NAG enrichment in liquid on the top and soil on the bottom). The most abundant bacterial (A) and fungal (B) phyla are shown over the 15-week incubation. The alpha diversities of bacteria (C) and fungi (D) were estimated using species richness and Simpson’s evenness.
- FIG 2
The influence of physical environment on taxonomic volatility, i.e., the tendency for the community to stabilize with respect to taxa being gained/lost over time. Each graph shows the weighted UniFrac distance for bacteria (A) and fungi (B) calculated between subsequent incubation times and plotted by weeks of incubation, dilution factor, and treatment conditions.
- FIG 3
The biological and physical variables correlated with beta diversity. Ordinations present canonical analysis of principal coordinates (CAP) of weighted UniFrac distances between samples. The percent variation captured by the vectors is shown on each axis. Each vector has a magnitude (length) and direction of a variable’s contributions to the principal components. Vectors represent respiration (CO2 per hour) (orange), volatility (red), and the most abundant phyla (purple).
Supplemental Material
FIG S1
The most abundant bacterial (A) and fungal (B) phyla are plotted for the native soil (labeled as “n”) and the 6-week-chitin-enriched soil (labeled as “c”). The alpha diversities of bacteria (C) and fungi (D) were estimated using species richness and Simpson’s evenness. Download FIG S1, PDF file, 0.01 MB.
Copyright © 2019 Zegeye et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
FIG S2
Rate of respiration varies over time based on dilution and physical environment. The respiration rate was plotted for both liquid (top) and soil (bottom) for NAG enrichment cultures from weeks 2 through 7 to avoid the light spike in CO2 released immediately after inoculation. Respiration was monitored three times a week with a PP Systems (Amesbury, MA) EGM-4 environmental gas monitor. The gas analyzer was set up in static mode to measure CO2 and monitor respiration in each sample at periodic intervals. To measure CO2, a 10-ml gas aliquot was taken from the incubation container using a sterile plastic syringe with a control valve. Download FIG S2, PDF file, 0.01 MB.
Copyright © 2019 Zegeye et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
FIG S3
Bacterial OTUs observed from 16S rRNA gene amplicon products in uninoculated controls. The most abundant OTUs shown in the controls were also found in the other samples. Download FIG S3, PDF file, 0.09 MB.
Copyright © 2019 Zegeye et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
TABLE S1
Final richness significantly varies between dilutions. Tukey’s honestly significant difference (HSD) test was performed to compare the richness between dilutions, and the P values are shown here. Download Table S1, DOCX file, 0.01 MB.
Copyright © 2019 Zegeye et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
FIG S4
Patterns of variation within sample groups and within replicates. (A) Beta dispersion (within sample group variation) varies among time points as shown by the pairwise permutation test for homogeneity of multivariate dispersion at each time. Pairwise comparisons of the observed P values are tabulated for both the 16S and ITS for liquid and soil treatment conditions and indicate significant difference of within group dispersion. (B) A linear regression fit to the pairwise distance between replicates also shows changes in dispersion over time. The different dilutions are shown in separate facets and are used as strata during the permutation testing of beta dispersion. Download FIG S4, PDF file, 2.9 MB.
Copyright © 2019 Zegeye et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
TABLE S2
The adonis test (permutational multivariate analysis of variance using distance matrices) was used to partition variation associated with dilution in the final time points from each treatment condition. The R2 column reports the percentage of weighted Unifrac distance that can be attributed to differing dilution. Download Table S2, DOCX file, 0.01 MB.
Copyright © 2019 Zegeye et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
TABLE S3
Outputs from the tests for homogeneity of multivariate dispersions performed with respect to each treatment. Download Table S3, DOCX file, 0.01 MB.
Copyright © 2019 Zegeye et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
