TABLE 1 

High-frequency mutationsa

GenePopulationPassageGenome positionNucleotide in sequence:Max frequencyAmino acid in sequence:Function
ReferenceMutantWTMutant
NCC301830080GA1.00Upstream of astC and xthA
NCC211830081CA0.74Upstream of astC and xthA
NCA272865605AG1.00Upstream of rpoS
acpPA271150972GA0.94MIAcyl carrier protein
acrAB30484803AT0.86LQTolC-AcrAB multidrug efflux pump
csgAB301103787GC0.90GRCurlin
cytRB154121654AG0.46SPDNA utilization negative regulator
cytRB274121864AC1.00YDDNA utilization negative regulator
cytRC154122103CT0.97GDDNA utilization negative regulator
cytRA214122140TG0.31TPDNA utilization negative regulator
lptDA2754939CT1.00CYLipopolysaccharide transporter
pitAA273635749AC1.00ILPi transporter
proQA61913507CG0.58APProline transport
putAC211077465AC0.87LRProline catabolism
rplFC303443879CT0.75RH50S ribosomal component
rpsLA303472555AC1.00LR30S ribosomal component
sspAB153374952AC0.48LRStringent starvation protein A
sspAC153375205CA0.97DYStringent starvation protein A
sspAC243375249CA0.83RLStringent starvation protein A
sspAB273375388CA0.96DYStringent starvation protein A
sucCB30763114CT0.90AVSuccinyl-CoA synthetase
sucCA27763213AG1.00DGSuccinyl CoA synthetase
tolCA273176519TG1.00ISTolC-AcrAB multidrug efflux pump
ybaLB30501117GT0.90S*Predicted Pi transporter
  • a NC, noncoding. The asterisk represents a stop codon/nonsense mutation.